King’s B agar. Pyoverdine, produced siderophore>to collect iron from the environment, glows under UV light. | image2 = Pseudomonas putida DIC image 400X.jpg| image2_caption = DIC image of Pseudomonas putida culture wet mount, 400X.| genus = Pseudomonas| species = putida| authority = Trevisan, 1889| type_strain = ATCC 12633 CCUG 12690 CFBP 2066 DSM 291 HAMBI 7 JCM 13063 and 20120 LMG 2257 NBRC 14164 NCAIM B.01634 NCCB 72006 and 68020 NCTC 10936

Bacillus fluorescens putidus” FlÃ¼gge 1886 Bacillus putidus Trevisan 1889 Pseudomonas eisenbergii Migula 1900 Pseudomonas convexa Chester 1901 Pseudomonas incognita Chester 1901 Pseudomonas ovalis Chester 1901 Pseudomonas rugosa (Wright 1895) Chester 1901 Pseudomonas striata Chester 1901 Pseudomonas mildenbergii David Hendricks Bergey>Bergey, et al. Arthrobacter siderocapsulatus Dubinina and Zhdanov 1975 Pseudomonas arvilla O. Hayaishi Pseudomonas barkeri Rhodes Pseudomonas cyanogena Hammer}}Pseudomonas putida is a Gram-negative, rod-shaped, saprophytic soil bacterium.BOOK,onlinelibrary.wiley.com/doi/book/10.1002/9781118960608, Bergey’s Manual of Systematics of Archaea and Bacteria, 2015-04-17, Wiley, 978-1-118-96060-8, Whitman, William B, 1, en, 10.1002/9781118960608.gbm01210, Rainey, Fred, KÃ¤mpfer, Peter, Trujillo, Martha, Chun, Jonsik, DeVos, Paul, Hedlund, Brian, Dedysh, Svetlana, It has a versatile metabolism and is amenable to genetic manipulation, making it a common organism used in research, bioremediation, and synthesis of chemicals and other compounds.The Food and Drug Administration (FDA) has listed P. putida strain KT2440 as Host-vector system safety level 1 certified (HV-1), indicating that it is safe to use without any extra precautions.JOURNAL, Kampers, Linde F. C., Volkers, Rita J. M., Martins dos Santos, Vitor A. P., 2019-06-14, Pseudomonas putida KT 2440 is HV 1 certified, not GRAS,dx.doi.org/10.1111/1751-7915.13443, Microbial Biotechnology, 12, 5, 845â€“848, 10.1111/1751-7915.13443, 31199068, 6680625, 1751-7915, Thus, use of P. putida in many research labs is preferable to some other Pseudomonas species, such as Pseudomonas aeruginosa, for example, which is an opportunistic pathogen.

History and phylogeny

Based on 16S rRNA analysis, P. putida was taxonomically confirmed to be a Pseudomonas species (sensu stricto) and placed, along with several other species, in the P. putida group, to which it lends its name.JOURNAL, Anzai, Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence, Int J Syst Evol Microbiol, 50, 4, 1563â€“89, Jul 2000, 10939664, Kim, H, Park, JY, Wakabayashi, H, Oyaizu, H, 10.1099/00207713-50-4-1563, etal, However, phylogenomic analysisJOURNAL, Keshavarz-Tohid, Vahid, Vacheron, Jordan, Dubost, Audrey, Prigent-Combaret, Claire, Taheri, Parissa, Tarighi, Saeed, Taghavi, Seyed Mohsen, MoÃ«nne-Loccoz, Yvan, Muller, Daniel, 2019-07-01, Genomic, phylogenetic and catabolic re-assessment of the Pseudomonas putida clade supports the delineation of Pseudomonas alloputida sp. nov., Pseudomonas inefficax sp. nov., Pseudomonas persica sp. nov., and Pseudomonas shirazica sp. nov,hal.archives-ouvertes.fr/hal-02392254/file/Pseudomonas%20putida%20revision%2015avril2019%20Yd.pdf, Systematic and Applied Microbiology, en, 42, 4, 468â€“480, 10.1016/j.syapm.2019.04.004, 31122691, 155282987, 0723-2020, free, JOURNAL, Nikolaidis, Marios, Mossialos, Dimitris, Oliver, Stephen G., Amoutzias, Grigorios D., 2020-07-24, Comparative Analysis of the Core Proteomes among the Pseudomonas Major Evolutionary Groups Reveals Species-Specific Adaptations for Pseudomonas aeruginosa and Pseudomonas chlororaphis, Diversity, en, 12, 8, 289, 10.3390/d12080289, 1424-2818, free, of complete genomes from the entire Pseudomonas genus clearly showed that the genomes that were named as P. putida did not form a monophyletic clade, but were dispersed and formed a wider evolutionary group (the putida group) that included other species as well, such as P. alkylphenolia, P. alloputida, P. monteilii, P. cremoricolorata, P. fulva, P. parafulva, P. entomophila, P. mosselii, P. plecoglossicida and several genomic species (new species which are not validly defined).JOURNAL, Keshavarz-Tohid, Vacheron, J, Dubost, A, Prigent-Combaret, C, Taheri, P, Tarighi, S, Taghavi, SM, MoÃ«nne-Loccoz, Y, Muller, D, etal, May 2019, Genomic, phylogenetic and catabolic re-assessment of the Pseudomonas putida clade supports the delineation of Pseudomonas alloputida sp. nov., Pseudomonas inefficax sp. nov., Pseudomonas persica sp. nov., and Pseudomonas shirazica sp. nov., Syst Appl Microbiol, 42, Pt 1, 468â€“480, 10.1016/j.syapm.2019.04.004, 31122691, 155282987, free, A variety of P. putida, called multiplasmid hydrocarbon-degrading Pseudomonas, is the first patented organism in the world. Because it is a living organism, the patent was disputed and brought before the United States Supreme Court in the historic court case Diamond v. Chakrabarty, which the inventor, Ananda Mohan Chakrabarty, won. It demonstrates a very diverse metabolism, including the ability to degrade organic solvents such as toluene.JOURNAL, 10.1111/j.1365-2958.1993.tb01222.x, 7934920, Transcriptional control of the Pseudomonas putida TOL plasmid catabolic pathways, 1993, MarquÃ©s, Silvia, Ramos, Juan L., Molecular Microbiology, 9, 5, 923â€“9, 20663917, This ability has been put to use in bioremediation, or the use of microorganisms to degrade environmental pollutants.

Genomics

The protein count and GC content of the (63) genomes that belong to the P. putida wider evolutionary group (as defined by a phylogenomic analysis of 494 complete genomes from the entire Pseudomonas genus) ranges between 3748â€“6780 (average: 5197) and between 58.7â€“64.4% (average: 62.3%), respectively. The core proteome of the analyzed 63 genomes (of the P. putida group) comprised 1724 proteins, of which only 1 core protein was specific for this group, meaning that it was absent in all other analyzed Pseudomonads.

Uses

Bioremediation

The diverse metabolism of wild-type strains of P. putida may be exploited for bioremediation; for example, it has been shown in the laboratory to function as a soil inoculant to remedy naphthalene-contaminated soils.JOURNAL, 16329969, 2005, Gomes, NC, Kosheleva, IA, Abraham, WR, Smalla, K, Effects of the inoculant strain Pseudomonas putida KT2442 (pNF142) and of naphthalene contamination on the soil bacterial community, 54, 1, 21â€“33, 10.1016/j.femsec.2005.02.005, FEMS Microbiology Ecology, free, Pseudomonas putida is capable of converting styrene oil into the biodegradable plastic PHA.WEB,www.livescience.com/605-immortal-polystyrene-foam-meets-enemy.html, Immortal Polystyrene Foam Meets its Enemy, Robert Roy, Britt, March 7, 2006, livescience.com, November 4, 2021, November 4, 2021,web.archive.org/web/20211104164356/https://www.livescience.com/605-immortal-polystyrene-foam-meets-enemy.html, live, JOURNAL, 16649270, 2006, Ward, PG, Goff, M, Donner, M, Kaminsky, W, O’Connor, KE, A two step chemo-biotechnological conversion of polystyrene to a biodegradable thermoplastic, 40, 7, 2433â€“7, Environmental Science & Technology, 10.1021/es0517668, 2006EnST...40.2433W, This may be of use in the effective recycling of polystyrene foam, otherwise thought to be not biodegradable.

Biocontrol

Pseudomonas putida has demonstrated potential biocontrol properties, as an effective antagonist of plant pathogens such as Pythium aphanidermatumJOURNAL, 11006841, 2000, Amer, GA, Utkhede, RS, Development of formulations of biological agents for management of root rot of lettuce and cucumber, 46, 9, 809â€“16, Canadian Journal of Microbiology, 10.1139/w00-063, and Fusarium oxysporum f.sp. radicis-lycopersici.JOURNAL, 17241352, 2007, Validov, S, Kamilova, F, Qi, S, Stephan, D, Wang, JJ, Makarova, N, Lugtenberg, B, Selection of bacteria able to control Fusarium oxysporum f. Sp. Radicis-lycopersici in stonewool substrate, 102, 2, 461â€“71, 10.1111/j.1365-2672.2006.03083.x, Journal of Applied Microbiology, 3098008, P. alloputida KT2440 genome“>

Oligonucleotide usage signatures of the P. alloputida KT2440 genome

Di- to pentanucleotide usage and the list of the most abundant octa- to tetradecanucleotides are useful measures of the bacterial genomic signature. The P. putida KT2440 chromosome is characterized by strand symmetry and intrastrand parity of complementary oligonucleotides. Each tetranucleotide occurs with similar frequency on the two strands. Tetranucleotide usage is biased by G+C content and physicochemical constraints such as base stacking energy, dinucleotide propeller twist angle, or trinucleotide bendability. The 105 regions with atypical oligonucleotide composition can be differentiated by their patterns of oligonucleotide usage into categories of horizontally acquired gene islands, multidomain genes or ancient regions such as genes for ribosomal proteins and RNAs. A species-specific extragenic palindromic sequence is the most common repeat in the genome that can be exploited for the typing of P. putida strains. In the coding sequence of P. putida, LLL is the most abundant tripeptide.BOOK, Cornelis, Pierre, Pseudomonas: Genomics and Molecular Biology, 1st, Caister Academic Press, 2008,www.horizonpress.com/pseudo, 978-1-904455-19-6, 2007-09-24, 2016-09-12,www.horizonpress.com/pseudo," title="web.archive.org/web/20160912181431www.horizonpress.com/pseudo,">web.archive.org/web/20160912181431www.horizonpress.com/pseudo, live, Phylogenomic analysis reclassified the strain KT2440 in a new species Pseudomonas alloputida.

Organic synthesis

Pseudomonas putida’s amenability to genetic manipulation has allowed it to be used in the synthesis of numerous organic pharmaceutical and agricultural compounds from various substrates.JOURNAL, Poblete-Castro, Ignacio, Becker, Judith, Dohnt, Katrin, dos Santos, Vitor Martins, Wittmann, Christoph, March 2012, Industrial biotechnology of Pseudomonas putida and related species,link.springer.com/10.1007/s00253-012-3928-0, Applied Microbiology and Biotechnology, en, 93, 6, 2279â€“2290, 10.1007/s00253-012-3928-0, 22350258, 10033/246536, 253775454, 0175-7598, 2023-02-19, 2023-03-15,web.archive.org/web/20230315121719/https://link.springer.com/article/10.1007/s00253-012-3928-0, live, free,

CBB5 and caffeine consumption

Pseudomonas putida CBB5, a nonengineered, wild-type variety found in soil, can live on caffeine and has been observed to break caffeine down into carbon dioxide and ammonia.WEB,blogs.scientificamerican.com/observations/newly-discovered-bacteria-lives-on-caffeine/, Newly Discovered Bacteria Lives on Caffeine, Katherine, Harmon, Scientific American Blog Network, 2021-11-04, 2021-11-04,web.archive.org/web/20211104164401/https://blogs.scientificamerican.com/observations/newly-discovered-bacteria-lives-on-caffeine/, live, JOURNAL, 20966097, 2011, Summers, RM, Louie, TM, Yu, CL, Subramanian, M, Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source, 157, Pt 2, 583â€“92, 10.1099/mic.0.043612-0, Microbiology, free,

References

External links

Risk Assessment Summary, CEPA 1999. www.bch.gc.ca/default.asp?lang=En&n=3CC8DDA3-1&xsl=bchdescriptor,showfull&xml=E1C5BCE8-FD3A-4A02-8F91-F47781EFDD32" title="web.archive.org/web/20131120135845www.bch.gc.ca/default.asp?lang=En&n=3CC8DDA3-1&xsl=bchdescriptor,showfull&xml=E1C5BCE8-FD3A-4A02-8F91-F47781EFDD32">Pseudomonas putida CR30RNSLL(pADPTel).
Pseudomonas putida is an example for plant growth promoting Rhizobacterium, which produces iron chelating substances.
Type strain of Pseudomonas putida at BacDive â€“ the Bacterial Diversity Metadatabase

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