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{{Short description|Chemical process of introducing a phosphate}}File:Phosporylation of a serine residue, before and after shot.png|thumb|SerineSerineIn biochemistry, phosphorylation is the attachment of a phosphate group to a molecule or an ion.BOOK, Betts, J. Gordon, Anatomy & physiology, 2013, OpenStax, 2.5 Organic compounds essential for human functioning, 978-1-947172-04-3,openstax.org/books/anatomy-and-physiology/pages/2-5-organic-compounds-essential-to-human-functioning, 16 April 2023, 2023-03-31,web.archive.org/web/20230331030005/https://openstax.org/books/anatomy-and-physiology/pages/2-5-organic-compounds-essential-to-human-functioning, live, This process and its inverse, dephosphorylation, are common in biology.JOURNAL, Chen J, He X, Jakovlić I, November 2022, Positive selection-driven fixation of a hominin-specific amino acid mutation related to dephosphorylation in IRF9, BMC Ecology and Evolution, 22, 1, 132, 10.1186/s12862-022-02088-5, 36357830, 9650800, 253448972, free, (File:CC-BY_icon.svg|50x50px) Text was copied from this source, which is available under a (creativecommons:by/4.0/|Creative Commons Attribution 4.0 International License). Protein phosphorylation often activates (or deactivates) many enzymes.JOURNAL, Oliveira AP, Sauer U, The importance of post-translational modifications in regulating Saccharomyces cerevisiae metabolism, FEMS Yeast Research, 12, 2, 104–117, March 2012, 22128902, 10.1111/j.1567-1364.2011.00765.x, free, JOURNAL, Tripodi F, Nicastro R, Reghellin V, Coccetti P, Post-translational modifications on yeast carbon metabolism: Regulatory mechanisms beyond transcriptional control, Biochimica et Biophysica Acta (BBA) - General Subjects, 1850, 4, 620–627, April 2015, 25512067, 10.1016/j.bbagen.2014.12.010, 10281/138736, free,

During respiration and photosynthesis

Phosphorylation is essential to the processes of both anaerobic and aerobic respiration, which involve the production of adenosine triphosphate (ATP), the “high-energy” exchange medium in the cell. During aerobic respiration, ATP is synthesized in the mitochondrion by addition of a third phosphate group to adenosine diphosphate (ADP) in a process referred to as oxidative phosphorylation. ATP is also synthesized by substrate-level phosphorylation during glycolysis. ATP is synthesized at the expense of solar energy by photophosphorylation in the chloroplasts of plant cells.

Phosphorylation of glucose

Glucose metabolism

Phosphorylation of sugars is often the first stage in their catabolism. Phosphorylation allows cells to accumulate sugars because the phosphate group prevents the molecules from diffusing back across their transporter. Phosphorylation of glucose is a key reaction in sugar metabolism. The chemical equation for the conversion of D-glucose to D-glucose-6-phosphate in the first step of glycolysis is given by:

Glycolysis

Glycolysis is an essential process of glucose degrading into two molecules of pyruvate, through various steps, with the help of different enzymes. It occurs in ten steps and proves that phosphorylation is a much required and necessary step to attain the end products. Phosphorylation initiates the reaction in step 1 of the preparatory stepBOOK,www.bioinfo.org.cn/book/biochemistry/chapt14/sim1.htm, Chapter 14: Glycolysis and the Catabolism of Hexoses, 2016-05-14, 2021-10-17,www.bioinfo.org.cn/book/biochemistry/chapt14/sim1.htm," title="web.archive.org/web/20211017002355www.bioinfo.org.cn/book/biochemistry/chapt14/sim1.htm,">web.archive.org/web/20211017002355www.bioinfo.org.cn/book/biochemistry/chapt14/sim1.htm, live, (first half of glycolysis), and initiates step 6 of payoff phase (second phase of glycolysis).BOOK, Biochemistry, Garrett R, Saunders College, 1995, Glucose, by nature, is a small molecule with the ability to diffuse in and out of the cell. By phosphorylating glucose (adding a phosphoryl group in order to create a negatively charged phosphate groupWEB, Hexokinase - Reaction,www.chem.uwec.edu/webpapers_f99/pages/Webpapers_F99/schneebm/Pages/reaction.html, 2020-07-29, www.chem.uwec.edu, 2020-12-02,web.archive.org/web/20201202023137/https://www.chem.uwec.edu/webpapers_f99/pages/Webpapers_F99/schneebm/Pages/reaction.html, live, ), glucose is converted to glucose-6-phosphate, which is trapped within the cell as the cell membrane is negatively charged. This reaction occurs due to the enzyme hexokinase, an enzyme that helps phosphorylate many six-membered ring structures. Phosphorylation takes place in step 3, where fructose-6-phosphate is converted to fructose 1,6-bisphosphate. This reaction is catalyzed by phosphofructokinase.While phosphorylation is performed by ATPs during preparatory steps, phosphorylation during payoff phase is maintained by inorganic phosphate. Each molecule of glyceraldehyde 3-phosphate is phosphorylated to form 1,3-bisphosphoglycerate. This reaction is catalyzed by glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The cascade effect of phosphorylation eventually causes instability and allows enzymes to open the carbon bonds in glucose.Phosphorylation functions is an extremely vital component of glycolysis, as it helps in transport, control, and efficiency.WEB,www.bachillerato.uchile.cl/files/Bioquimica/glycolysis/glyintro/page07.htm, Maber J, Introduction to Glycolysis, 18 November 2017, 6 April 2017,www.bachillerato.uchile.cl/files/Bioquimica/Glycolysis/glyintro/page07.htm," title="web.archive.org/web/20170406210528www.bachillerato.uchile.cl/files/Bioquimica/Glycolysis/glyintro/page07.htm,">web.archive.org/web/20170406210528www.bachillerato.uchile.cl/files/Bioquimica/Glycolysis/glyintro/page07.htm, dead,

Glycogen synthesis

Glycogen is a long-term store of glucose produced by the cells of the liver. In the liver, the synthesis of glycogen is directly correlated with blood glucose concentration. High blood glucose concentration causes an increase in intracellular levels of glucose 6-phosphate in the liver, skeletal muscle, and fat (adipose) tissue. Glucose 6-phosphate has role in regulating glycogen synthase.High blood glucose releases insulin, stimulating the translocation of specific glucose transporters to the cell membrane; glucose is phosphorylated to glucose 6-phosphate during transport across the membrane by ATP-D-glucose 6-phosphotransferase and non-specific hexokinase (ATP-D-hexose 6-phosphotransferase).JOURNAL, Villar-Palasí C, Guinovart JJ, The role of glucose 6-phosphate in the control of glycogen synthase, FASEB Journal, 11, 7, 544–558, June 1997, 9212078, 10.1096/fasebj.11.7.9212078, free, 2789124, Liver cells are freely permeable to glucose, and the initial rate of phosphorylation of glucose is the rate-limiting step in glucose metabolism by the liver.JOURNAL, Walker DG, Rao S, The role of glucokinase in the phosphorylation of glucose by rat liver, The Biochemical Journal, 90, 2, 360–368, February 1964, 5834248, 1202625, 10.1042/bj0900360, The liver’s crucial role in controlling blood sugar concentrations by breaking down glucose into carbon dioxide and glycogen is characterized by the negative Gibbs free energy (ΔG) value, which indicates that this is a point of regulation with.{{clarify|date=January 2023}} The hexokinase enzyme has a low Michaelis constant (K{{sub|m}}), indicating a high affinity for glucose, so this initial phosphorylation can proceed even when glucose levels at nanoscopic scale within the blood.The phosphorylation of glucose can be enhanced by the binding of fructose 6-phosphate (F6P), and lessened by the binding fructose 1-phosphate (F1P). Fructose consumed in the diet is converted to F1P in the liver. This negates the action of F6P on glucokinase,JOURNAL, Walker DG, Rao S, The role of glucokinase in the phosphorylation of glucose by rat liver, The Biochemical Journal, 90, 2, 360–368, February 1964, 5834248, 1202625, 10.1042/bj0900360, which ultimately favors the forward reaction. The capacity of liver cells to phosphorylate fructose exceeds capacity to metabolize fructose-1-phosphate. Consuming excess fructose ultimately results in an imbalance in liver metabolism, which indirectly exhausts the liver cell’s supply of ATP.WEB,cmgm.stanford.edu/biochem200/regulation/, Regulation of Glycolysis, cmgm.stanford.edu, 2017-11-18, 2009-03-03,cmgm.stanford.edu/biochem200/regulation/," title="web.archive.org/web/20090303224811cmgm.stanford.edu/biochem200/regulation/,">web.archive.org/web/20090303224811cmgm.stanford.edu/biochem200/regulation/, dead, Allosteric activation by glucose 6-phosphate, which acts as an effector, stimulates glycogen synthase, and glucose 6 phosphate may inhibit the phosphorylation of glycogen synthase by cyclic AMP-stimulated protein kinase.

Other processes

Phosphorylation of glucose is imperative in processes within the body. For example, phosphorylating glucose is necessary for insulin-dependent mechanistic target of rapamycin pathway activity within the heart. This further suggests a link between intermediary metabolism and cardiac growth.JOURNAL, Sharma S, Guthrie PH, Chan SS, Haq S, Taegtmeyer H, Glucose phosphorylation is required for insulin-dependent mTOR signalling in the heart, Cardiovascular Research, 76, 1, 71–80, October 2007, 17553476, 2257479, 10.1016/j.cardiores.2007.05.004,

Protein phosphorylation

Protein phosphorylation is the most abundant post-translational modification in eukaryotes. Phosphorylation can occur on serine, threonine and tyrosine side chains (often called ‘residues’) through phosphoester bond formation, on histidine, lysine and arginine through phosphoramidate bonds, and on aspartic acid and glutamic acid through mixed anhydride linkages. Recent evidence confirms widespread histidine phosphorylation at both the 1 and 3 N-atoms of the imidazole ring.JOURNAL, Fuhs SR, Hunter T, pHisphorylation: the emergence of histidine phosphorylation as a reversible regulatory modification, Current Opinion in Cell Biology, 45, 8–16, April 2017, 28129587, 5482761, 10.1016/j.ceb.2016.12.010, JOURNAL, Fuhs SR, Meisenhelder J, Aslanian A, Ma L, Zagorska A, Stankova M, Binnie A, Al-Obeidi F, Mauger J, Lemke G, Yates JR, Hunter T, 6, Monoclonal 1- and 3-Phosphohistidine Antibodies: New Tools to Study Histidine Phosphorylation, Cell, 162, 1, 198–210, July 2015, 26140597, 4491144, 10.1016/j.cell.2015.05.046, Recent work demonstrates widespread human protein phosphorylation on multiple non-canonical amino acids, including motifs containing phosphorylated histidine, aspartate, glutamate, cysteine, arginine and lysine in HeLa cell extracts.JOURNAL, Hardman G, Perkins S, Brownridge PJ, Clarke CJ, Byrne DP, Campbell AE, Kalyuzhnyy A, Myall A, Eyers PA, Jones AR, Eyers CE, 6, Strong anion exchange-mediated phosphoproteomics reveals extensive human non-canonical phosphorylation, The EMBO Journal, 38, 21, e100847, October 2019, 31433507, 6826212, 10.15252/embj.2018100847, free, However, due to the chemical lability of these phosphorylated residues, and in marked contrast to Ser, Thr and Tyr phosphorylation, the analysis of phosphorylated histidine (and other non-canonical amino acids) using standard biochemical and mass spectrometric approaches is much more challengingJOURNAL, Gonzalez-Sanchez MB, Lanucara F, Hardman GE, Eyers CE, Gas-phase intermolecular phosphate transfer within a phosphohistidine phosphopeptide dimer, International Journal of Mass Spectrometry, 367, 28–34, June 2014, 25844054, 4375673, 10.1016/j.ijms.2014.04.015, 2014IJMSp.367...28G, JOURNAL, Gonzalez-Sanchez MB, Lanucara F, Helm M, Eyers CE, Attempting to rewrite History: challenges with the analysis of histidine-phosphorylated peptides, Biochemical Society Transactions, 41, 4, 1089–1095, August 2013, 23863184, 10.1042/bst20130072, and special procedures and separation techniques are required for their preservation alongside classical Ser, Thr and Tyr phosphorylation.BIORXIV, Hardman G, Perkins S, Ruan Z, Kannan N, Brownridge P, Byrne DP, Eyers PA, Jones AR, Eyers CE, Extensive non-canonical phosphorylation in human cells revealed using strong-anion exchange-mediated phosphoproteomics, 2017, 10.1101/202820, The prominent role of protein phosphorylation in biochemistry is illustrated by the huge body of studies published on the subject (as of March 2015, the MEDLINE database returns over 240,000 articles, mostly on protein phosphorylation).

See also

• Moiety conservation
• Phosida
• Phosphoamino acid analysis
• Phospho3D

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References

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External links

• natureprotocols.com/2007/01/10/functional_analyses_for_sitesp.php" title="web.archive.org/web/20090416125554natureprotocols.com/2007/01/10/functional_analyses_for_sitesp.php">Functional analyses for site-specific phosphorylation of a target protein in cells (A Protocol)

{{Protein posttranslational modification}}{{Authority control}}